NGS Scientist

About the Company

Come join the Cardiometabolic Research (CMR) team at ***! We are focused on the discovery and early phase clinical development of biologic, small molecule, oligonucleotide, and cell therapies for treatment of diabetes, obesity, cardiovascular disease, and associated metabolic disorders. Client is an EEO/Affirmative Action Employer and does not discriminate on the basis of age, race, color, religion, gender, sexual orientation, gender identity, gender expression, national origin, protected veteran status, disability or any other legally protected status. Being on a temporary contractor assignment at Client means working in an innovative culture where you have the opportunity to support the work of thousands of others around the globe dedicated to improving the lives of others. Client discovers and brings life-changing medicines to those who need them, improves the understanding and management of disease, and gives back to communities through philanthropy and volunteerism. *** passionately promotes food security and fosters the human-animal bond to enrich the lives of its customers and the customers they serve as well. Our values: integrity, excellence, respect for people. Greater than 90% of workers rate their Client assignment as above average or excellent.

About the Role

The Data Science team within CMR is seeking someone with wide-ranging experience in next-generation sequencing library preparation to help achieve our research goals. The successful candidate will be responsible for RNA-seq, scRNA-seq, snRNA-seq, and other types of -seq library preparations for all CMR groups. This will be a cross-therapeutic area role encompassing opportunities to impact science in the Boston team.

Responsibilities

• Conduct experiments to identify and validate drug targets.
• Support assay design, optimization, validation, and data analyses.
• Design and execute molecular and cell biology experiments.
• Troubleshoot and analyze complex data in support of drug discovery projects.
• Presentation of results and conclusions both orally and written.

Qualifications

• B.S. and/or M.S. in biological or biochemical sciences (or a related field) with 3-5 years of laboratory research experience.

Required Skills

• Molecular biology mastery of nucleic acid extraction and purification, quantification, NGS library preparation/QC, and qPCR.
• Strong background in NGS technologies and their applications, including quantification, sequencing, and data QC (preferably Illumina).
• Direct experience with RNA-seq (bulk) and amplicon-seq.
• Direct experience with single-cell and single-nucleus RNA-seq library preparation is required. Candidates must be familiar with multiple scRNA-seq/snRNA-seq platforms (e.g., 10X Genomics Chromium, Illumina PIP-seq, ScaleBio, or Parse Biosciences).

Preferred Skills

• Familiarity with CRISPR-based gene editing and high-throughput screening is a plus.
• Experience with automation is a plus (Mantis, Integra systems, Liquid Handlers, SPTLabtech Firefly, Tecan, etc).
• Strong organizational, time management, and communication skills.
• Experience with documentation in electronic notebook software.